ABSTRACT
Objective To investigate the recovery mechanism of brain function in patients with mo-tor aphasia after cerebral infarction by resting-state functional magnetic resonance imaging (fMRI) functional connectivity. Methods 10 patients with aphasia after cerebral infarction (aphasia group),and 10 patients without aphasia (control group)in the same period were enrolled.Both patients underwent resting-state fMRI examination.Patients with aphasia received a second fMRI examination 1 month later.The SPM8 software and DPARSF software were used to process the data.The back of the left middle frontal gyrus ( LFMG) was se-lected as the seed point for functional connectivity analysis.REST was used for pairing and two-sample t-tests. Results Compared with the control group,the brain regions with increased LMFG-ROI functional connectiv-ity were right insula(MNI:x,y,z:45,12,0,t＝7.98),right inferior frontal gyrus (triangular,ankle,island cap) (MNI:x,y,z:42,6,27,t＝6.75),right upper temporal gyrus and right middle temporal gyrus( MNI:x, y,z:48,－45,6,t＝10.57),right superior border gyrus(MNI:x,y,z:15,－66,60,t＝5.59) and right angle gy-rus(MNI:x,y,z:54,－50,12,t＝9.55) in the aphasia group (before rehabilitation),and the brain regions with reduced functional connectivity were left posterior cingulate gyrus( MNI:x,y,z:6,－75,9,t＝－10.05), and left anterior wedge lobe(MNI:x,y,z:－6,－69,33,t＝－9.07).Compared with the control group,the brain regions with enhanced LMFG-ROI functional connectivity in the aphasia group (after rehabilitation 1 month) included head of left caudate nucleus,left middle frontal gyrus and inferior frontal gyrus,left globus pallidus, left central anterior gyrus,central posterior gyrus,left insula; the brain regions with reduced functional con-nectivity were right hippocampus,left cerebellum,right lingual gyrus,posterior left cingulate gyrus,right oc-cipital lobe and right anterior wedge lobe.Compared with aphasia group before rehabilitation,the brain regions with increased LMFG-ROI functional connectivity after rehabilitation 1 month were the left frontal frontal gy-rus(MNI:x,y,z:－51,15,24),t＝15.87),left frontal parietal island cover(MNI:x,y,z:－24,－66,42,t＝5.20),left central anterior gyrus and central posterior gyrus(MNI:x,y,z:－15,－16,55,t＝6.53); and the reduced brain regions were the right superior temporal gyrus and middle temporal gyrus(MNI:x,y,z:57,－18,30,t＝－15.21),right insula (MNI:x,y,z:－24,－66,42,t＝－5.20)and right superior border gyrus (MNI:x,y,z:15,－66,60,t＝－7.69). Conclusion The functional reorganization of the brain regions around the left hemisphere's injury lingual area may be the main mechanism of brain functional plasticity in patients with aphasia after cerebral infarction,and the right hemisphere is also involved in this process.In both the a-cute and chronic phases of motor aphasia,activation of the posterior left cingulate gyrus is reduced.
ABSTRACT
Objective To know of the concerning conditions of health information ,the ways to get health information ,and its influence factors among young people .Methods The health information seeking behaviors of people aged from 18 to 44 were inves-tigated by online electronic questionnaire and paper questionnaire from March to April in 2014 .Results 502 valid questionnaires were collected ,including 46 .81% men and 53 .19% women ,mean age was (29 .77 ± 6 .27)years old .40 .96% people were often concerned about the health information ,and 82 .39% people got health information through two or more ways ,the select frequency of the various ways were as follows :computer (77 .20% ) ,television (60 .3% ) ,relatives and friends (58 .70% ) ,mobile phones (54 .87% ) ,newspapers ,magazines ,books (53 .20% ) ,radio (9 .26% )and others 4 .80 % .Gender ,education ,occupation ,medical ed-ucation background ,all of them have effect on the ways of obtained health information ,and the effect of knowledge on the selection of internet pathways was greatest .Conclusion The internet has become the most major ways for people to obtain health informa-tion .The ways to obtain health information are affected by multiple factors ,so relative department should provide some convenient channels according to the characters of each group to get health information .
ABSTRACT
Objective To determine the levels of miR-193a-3p in serum from patients with renal clear cell carcinoma,and eval-uate the potential of miR-193a-3p asa molecular biomarker of renal clear cell carcinoma.Methods Serum samples were taken from 107 untreated hospitalized patients with renal clear cell carcinoma (TNM Ⅰ grade 76 cases,Ⅱ grade 16 cases,Ⅲ grade 2 cases,Ⅳ grade 8 cases and unknown 5 cases)in Nanjing General Hospital from 2010 to 2014 year and 107 age-and gender-matched healthy individuals to determine the level of miR-193a-3p by quantitative reverse-transcription PCR (qRT-PCR). The early diagnostic value of miR-193a-3p for renal clear cell carcinoma was assessed by ROC curve.Results qRT-PCR confirmed that serum miR-193a-3p expression levels was significantly elevated in cancer than in normal controls (3.294± 1.526 vs 1.944±0.600,P =0.000).TNM Ⅰ grade (3.411±1.676 vs 1.944±0.600,P =0.000),Ⅱ grade (2.926±0.927 vs 1.944±0.600,P =0.001),Ⅳ grade(2.926±0.894 vs 1.944±0.600,P =0.000)is significantly higher than that in con-trol group.The area under the ROC curve (AUC)of miR-193a-3p was 0.820 (95% CI,0.756 ~ 0.883),demonstratinga high sensitivity (80.3%)and specificity (93.5%)for the early diagnosis of renal clear cell carcinoma.Conclusion MiR-193a-3p content was significantly elevated in serum from renal clear cell carcinoma and has a high accuracy in diagnosing ear-ly cancer,which holds potential as molecular biomarker for renal clear cell carcinoma.